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KMID : 0043320100330081261
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Archives of Pharmacal Research
2010 Volume.33 No. 8 p.1261 ~ p.1267
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An experimental study of rabbit conjunctival epithelial toxicity using co-treatment with Mitomycin-C and a histone deacetylase inhibitor
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Kim Tae-Hyun
Oh Hyun-Cheol
Yoo Young-Hyun
Rho Jee-Hyun
Park Woo-Chan
Ahn Hee-Bae
Jeung Woo-Jin
Rho Sae-Heun
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Abstract
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This study was conducted to evaluate cytotoxicity due to co-treatment with low-dose Mitomycin-C (MMC) and the histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA) for glaucoma filtration surgery. In this study, the effect of co-treatment with MMC and SAHA to induce apoptosis in cultured conjunctival epithelial cells (CEs) in rabbit was investigated. The cytotoxic potential following co-treatment with MMC and SAHA in CEs via assay for reactive oxygen species (ROS) and lactate dehydrogenase (LDH) was also examined. Co-treatment with MMC and SAHA did not induce apoptosis in CEs. In addition, co-treatment with MMC and SAHA caused no significant alteration of ROS and LDH levels in CEs. This study therefore shows that low-dose MMC could still be used as an antimetabolite in co-treatment with SAHA to effectively inhibit fibrosis of Tenon’s capsule fibroblasts (TCFs) following glaucoma filtration surgery, while minimizing cytotoxicity in CEs.
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KEYWORD
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Mitomycin-C, Histone deacetylase inhibitor, Suberoylanilide hydroxamic acid (SAHA), Apoptosis, Glaucoma filtration surgery
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